ABSTRACT
This research was aimed to evaluate the protective effect and potential mechanism of Yiqi Tongluo Particles(YQTLs).Firstly,an animal model of multiple cerebral infarction(MCI) with Qi deficiency and blood stasis was established.Rats were randomly divided into six groups:SHAM group,Vehicle group,Buyang Huanwu decoction original group(BYHWO),EGb761 group,high and low dose of YQTLs group.Rats underwent sleep deprivation after one week of MCI and the tongues and pulses of rats after six weeks of sleep deprivation were detected,followed by collecting blood to analysis the blood coagulation.Differential expression of angiogenesis associated proteins was examined using proteomic research and verified by immunohistochemical.RESULTS: showed that neurological function score was obviously declined,G and B value of tongue surface was increased significantly and the pulse distension,the activated partial thromboplatin time(APTT) as well as prothrombin time(PT) were recovered following YQTLs 7.56 g·kg-1 treatment.Furthermore,G value of tongue surface,APTT and PT were also improved by YQTLs 3.78 g·kg-1.The results of proteomic technology showed that proteins associated with angiogenesis were reversed compared with Vehicle group.Moreover,the expression of VEGFR2 from immunohistochemical was promoted after YQTLs treatment.The MCI with Qi deficiency and blood stasis was alleviated obviously following YQTLs treatment and the possible mechanism was that YQTLs may enhance angiogenesis during cerebral ischemia.
Subject(s)
Animals , Rats , Angiogenesis Inducing Agents , Pharmacology , Cerebral Infarction , Drug Therapy , Drugs, Chinese Herbal , Pharmacology , Proteomics , Qi , Random AllocationABSTRACT
The comparison on evaluating blood stasis syndrome in sleep deprived rats was carried out by using R, G, B image analysis of Tongue and palm as well as auricle, palm surface laser Doppler flow perfusion. The experiment was performed by means of a small platform on the water environment for sleep deprivation. The rats were weekly weighed at fixed time, and their macroscopic signs were observed; and their tongue and palm images of the control and model group were respectively collected by the SLR camera at the 2nd, 4th and 6th week. Then the color saturation analysis was performed by means of proofreading with the standard colorimetric card. At the same time, the laser dopper flowmetry was used to analyze the perfusion of auricle and foot flow in rats. It turned out that there was no significant difference in the R,G,B value of the tongue and palm in rats between normal group and model group at the first stage(at the 2nd week), so were the perfusion of auricle and foot flow in rats. But at the second stage (at the 4th week), the R value of tongue in model group rats was obviously lower than that in normal group(<0.01), and the other value (G,B) of tongue in module rats had a decease tendency, but there was no statistical significance. However, the perfusion of left and right auricle flow in model group rats were dramatically decreased as compared with the normal group(<0.01); there was still no significant difference in the perfusion of the palm between two groups. It was found that R,G,B value in model group had a lower trend as compared with the control group of the tongue and palm images at the third stage (at the 6th week), but no statistically significant difference. The perfusion of left and right auricle flow in model group was constantly decreased as compared with the normal group(<0.01).Right and left foot blood flow was lower than the normal group, but no statistically significant difference. We can safely conclude that the results of the R, G, B values of the tongue in rats could objectively reflect the characteristics of the rats with blood stasis syndrome, which were consistent with the diagnosis of clinical tongue image. As a method of microcirculation evaluation, the surface laser doppler perfusion of auricle can exhibit the characteristics of blood stasis in model rats, but also was more objective and reproducible. Therefore, the combination of R, G, B value of tongue as well as auricle laser doppler blood flow is more beneficial to the objective evaluation of index in the later study of traditional Chinese medicine blood stasis syndrome model.
ABSTRACT
Blood stasis syndrome is the pre-state of thrombotic disease. The model of blood stasis syndrome in rats was induced by sleep deprivation to study on effects of blood stasis syndrome on platelet activation. The weight, the color of tongue and hemorheology for the blood stasis syndrome of Chinese medicine were measured after modeling. The release of platelet granules and platelet activation factors in plasma were detected by ELISA kit related indicators to provide experimental basis for platelet function evaluation and related drug effects in syndrome research. The results showed that the weight of the model group rats was significantly lower than that of the normal group (<0.01). The tongue showed a dark purple blood stasis pattern, and the R, G and B values of the tongue surface in model group were significantly lower than those of the normal group (<0.01). The hemorheological parameters including high shear, middle shear and low shear viscosity in whole blood were significantly higher than those in the normal group (<0.01). But plasma viscosity did not change significantly. The release levels of platelet α particles (GMP-140, -TG, PF4) and dense particles (ADP, 5-HT) were significantly higher than those in the normal group (<0.01). The levels of TXB₂ and 6-keto-PGF₁α in plasma were significantly higher than those in the normal group (<0.01). The ratios of TXB₂ and 6-keto-PGF₂α were also significantly higher than those in the normal group (<0.01). The levels of PAF in plasma in model group were significantly higher than those in the normal group (<0.01). It was concluded that platelet functions could be changed induced by sleep deprivationin rats with blood stasis syndrome, and there might be inflammation and endothelial cell dysfunction.
ABSTRACT
This study was designed to investigate the effect of Yinxing Mihuan oral solution on rats with myocardial ischemia injury. Left anterior descending (LAD) coronary artery was occluded in rats to establish the model. Yinxing Mihuan oral solution was given by intragastric administration daily for one week at dosage of 309 and 618 mg·kg-1. Cardiac ultrasound function, pathologic change and serum myocardial enzymes were determined to evaluate the effect of Yinxing Mihuan oral solution. The heart function was significantly reduced in the model group compared with sham operation group, and the pathologic damage was clear. The changes were significantly improved by Diltiazem hydrochloride tablets in heart function and ejection fraction (P -1) (P P < 0.01). In addition, Yinxing Mihuan oral solution decreased the myocardial damage and inhibited inflammatory reaction, and inhibited platelet activation factor. Yinxing Mihuan oral solution can protect against myocardial ischemia injury, inflammation and platelet activation in the rat model.